of interactions between preimplantation bovine embryos and
uterine epithelial cells
During the first
funding period we were focused on embryo-induced
alterations in the bovine endometrium during the
pre-attachment period (Day 18). Using modern holistic
proteome analysis, endometrium samples derived from
pregnant and non-pregnant animals as well as samples of
endometrium/embryo co-culture experiments were
investigated (Berendt et al.). To allow a fast and
effective validation of our candidate proteins as well as
the validation of candidates found by project partners, a
set of antibodies was generated and characterized. These
antibodies allow a fast and effective detection and
quantification of candidate proteins in a higher number of
samples and enable their immunhistochemical localization.
During the second funding period we will analyze
endometrium samples from additional stages of gravidity
using quantitative LC-MS/MS methods to get a kinetic view
of alteration in the endometrium proteome during early
gravidity. In a second approach we will make use of
recently introduced ultrasensitive fluorophores (DIGE
saturation labels) and expand our investigations to the
bovine embryo proteome.
In detail our work program includes:
a) Comparative proteome analysis of
four different development stages of blastocytes.
b) LC-MS based analysis of
endometrium proteomes during early gravidity.
c) Investigation of Interferon tau
induced alterations in the bovine endometrium using stable
isotope labelling and LC-MS techniques.
d) Targeted validation of protein
candidates using antibodies generated in the first funding
Berendt FJ, Fröhlich T,
Schmidt SEM, Reichenbach HD, Wolf E, Arnold GJ (2005)
Holistic differential analysis of embryo-induced
alterations in the proteome of bovine endometrium in the
pre-attachment period. Proteomics 5, 2551-2560
Return to the
projects main page.